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Promega
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Image Search Results
Journal: Kidney international
Article Title: Imaging mass spectrometry reveals direct albumin fragmentation within the diabetic kidney
doi: 10.1016/j.kint.2018.01.040
Figure Lengend Snippet: Examples of albumin fragment localization in diabetic renal tubules. PAS staining (right) and IMS (left) were performed on the same section; immunostaining and IMS were performed on serial sections. The resulting immunofluorescence and IMS images were co-registered. (A) Albumin fragments are not detected in glomeruli (marked by white-dashed circles). (B) Albumin fragments are localized to renal tubules. An example IMS ion image of the albumin peptide 25-48 acquired at 10 μm spatial resolution with corresponding H&E stain. After the tissue was imaged, the matrix was removed with a series of ethanol washes and the tissue was H&E stained. A zoomed in region is displayed in the right panel for clarity. (C) Albumin fragments (Left) co-localize with Lotus tetragonolobus lectin (LTL), a proximal tubule marker (Right). (D) Dolichos biflorus agglutinin (DBA), a distal tubule and collecting duct marker (Right) co-localizes with albumin fragments (Left). Scale bars for panels A, C and D = 200 μm. Scale bars for panel B= 50 μm (top panel) and 20 μm (bottom panel).
Article Snippet: Immunostaining Fluorescein-labeled lotus tetragonolobus lectin (LTL) and fluorescein-labeled
Techniques: Staining, Immunostaining, Immunofluorescence, Marker
Journal: Diabetes
Article Title: Dissecting Human Gene Functions Regulating Islet Development With Targeted Gene Transduction
doi: 10.2337/db15-0042
Figure Lengend Snippet: Antibodies used in this study
Article Snippet:
Techniques: Plasmid Preparation